Leeds Institute of Cancer and Pathology

Pre-Cancer Genomics

Catherine Daly – Research Officer

 

Catherine_02062010

Catherine Daly
Research Officer
Pre-Cancer Genomics Group
Leeds Institue of Cancer and Pathology
Wellcome Trust Brenner Building
St James University Hospital
Becketts Street
Leeds
LS9 7TF

Tel 0113 3438417
Email C.L.Daly@leeds.ac.uk

 

Role – Research Officer

My main role within the Pre-Cancer Genomics Group is to perform assays that validate the group’s findings from the whole genome sequencing and copy number assays we run on a wide range of clinical samples. To date, this has included PCR, RT PCR and most recently Agilent’s Haloplex Target Enrichment. We have collected a wide range of sample types, some difficult to process and some in minute quantities so a significant degree of optimisation is required. I also oversee our NGS pipeline for both our clinical samples, and those of our collaborators. This includes the training and supervision of students and technical staff involved in the preparation of samples for NGS and communication with collaborators with regards to experimental design and results.

I am also responsible for the implementation of the Human Tissue Act guidelines for all the PCG tissue collections, with the aim of maintaining HTA compliance. To date, this has included the design and administration of our tissue tracking databases, document control systems and sample collection and processing SOPs.

Publications

Stead LF, Berri S, Wood HM, Egan P, Conway C, Daly C, Papagiannopoulos K, Rabbitts P. (2012). The transcriptional consequences of somatic amplifications, deletions and rearrangements in a human lung squamous cell carcinoma. Neoplasia 14(11): 1075-86

Belvedere O, Berri S, Chalkley R, Conway C, Barbone F, Pisa F, MacKlennan K, Daly C, Alsop M, Morgan J, Menis J, Tcherveniakov P, Papgiannopoulos K, Rabbitts P, Wood HM. (2012). A computational index derived from whole-genome copy number analysis is a novel tool for prognosis in early stage lung squamous cell carcinoma. Genomics 99: 18-24

Richman SD, Chambers P, Seymour MT, Daly C, Grant S, Hemmings G and Quirke P. (2011). Intra-tumoral heterogeneity of KRAS and BRAF mutation status in patients with advanced colorectal cancer (aCRC) and cost-effectiveness of multiple sample testing. Analytical Cellular Pathology, 34 : 61-66

Wood H, Belvedere O, Conway C, Daly C, Chalkley R, Bickerdike M, McKinley C, Egan P, Ross L, Hayward B, Morgan J, Davidson L, MacKlennan K, Ong TK, Papgiannopoulos K, Cook I, Adams DJ, Taylor GR and Rabbitts P. (2010). Using next-generation sequencing for high resolution multiplex analysis of copy number variation from nanogram quantities of DNA from formalin-fixed paraffin-embedded specimens. Nucleic Acids Research, 38(14) 1-11

Richman S, Seymour MT, Chambers P, Elliott F, Daly CL, Meade AM, Taylor G, Barrett JH and Quirke P. (2009) KRAS and BRAF Mutations in Advanced Colorectal Cancer Are Associated with Poor Prognosis but do not Preclude Benefit from Oxaliplatin or Irinotecan: Results From the MRC FOCUS Trial. Journal of Clinical Oncology, Vol. 27(35) 5931-5937

Braun MS, Richman SD, Thompson L, Daly CL, Meade A, Adlard JW, Allen JM, Parmar M, Quirke P, Seymour MT. (2009) Association of Molecular Markers with Toxicity Outcomes in a Randomised Trial of Chemotherapy for Advanced Colorectal Cancer: The FOCUS Trial. Journal of Clinical Oncology, Vol 27(33) 5519-5528

Braun MS, Richman SD, Quirke P, Daly C, Adlard JW, Elliott F, Barrett JH, Selby P, Meade A, Stephens R, Parmar M and Seymour MT. (2008) Predictive Biomarkers of Chemotherapy Efficacy in Colorectal Cancer: Results from the UK MRC Focus Trial. Journal of Clinical Oncology, Vol 26(16) 2690-2698

Vakurov A, Simpson CE, Daly CL, Gibson TD, Millner PA. (2005) Acetylecholinesterase-based biosensor electrodes for organophosphate pesticide detection II. Immobilization and stabilization of acetylecholinesterase. Biosensors and Bioelectronics 20: 2324–2329

Vakurov A, Simpson CE, Daly CL, Gibson TD, Millner PA (2004) Acetylcholinesterase-based biosensor electrodes for organophosphate pesticide detection I. Modification of carbon surface for immobilization of acetylcholinesterase. Biosensors and Bioelectronics 20: 1118–1125

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